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| Palmieri,Darío Abel; Novelli,Valdenice Moreira; Bastianel,Marinês; Cristofani-Yaly,Mariângela; Astúa-Monge,Gustavo; Carlos,Eduardo Fermino; Oliveira,Antonio Carlos de; Machado,Marcos Antonio. |
| Nearly 65,000 citrus EST (Expressed Sequence Tags) have been investigated using the CitEST project database. Microsatellites were investigated in the unigene sequences from Citrus spp. and Poncirus trifoliata. From these sequences, approximately 35% of the non-redundant ESTs contained SSRs. The frequencies of different SSR motifs were similar between Citrus spp and trifoliate orange. In general, mononucleotide repeats appeared to be the most abundant SSRs in the CitEST database, but we also identify di-, tri-, tetra-, penta- and hexanucleotide repeats. The AG/CT and AAG/CTT were the most common dinucleotide and trinucleotide motifs, with frequencies of 54.4% and 25.2%, respectively. Primer sequences flanking SSR motifs were successfully designed and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus spp.; Poncirus trifoliata; ESTs; Microsatellites; In silico polymorphism. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500029 |
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